Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase NS5
Molecule details ›
Chain: C
Length: 36 amino acids
Theoretical weight: 4.32 KDa
Source organism: Dengue virus 2 Puerto Rico/PR159-S1/1969
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 36 amino acids
Theoretical weight: 4.32 KDa
Source organism: Dengue virus 2 Puerto Rico/PR159-S1/1969
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P12823 (Residues: 3353-3388; Coverage: 1%)
Importin subunit alpha-1
Molecule details ›
Chain: E
Length: 427 amino acids
Theoretical weight: 46.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Length: 427 amino acids
Theoretical weight: 46.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P52293 (Residues: 71-497; Coverage: 81%)
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup:
P212121
Expression system: Escherichia coli BL21(DE3)
