› 5hhn

X-ray diffraction
2.03Å resolution

Crystal Structure of HLA-A*0201 in complex with M1-F5L

Released:
DOI: 10.2210/pdb5hhn/pdb
PDB entry 5hhn coloured by chain, front view.
PDB entry 5hhn coloured by chain, side view.
PDB entry 5hhn coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for universal HLA-A*0201-restricted CD8+ T-cell immunity against influenza viruses.
Valkenburg SA, Josephs TM, Clemens EB, Grant EJ, Nguyen TH, Wang GC, Price DA, Miller A, Tong SY, Thomas PG, Doherty PC, Rossjohn J, Gras S, Kedzierska K
Proc Natl Acad Sci U S A 113 4440-5 (2016)
PMID: 27036003

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137785 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
M1-F5L, GILGLVFTL Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 932 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 53.773Å b: 80.202Å c: 57.445Å
α: 90° β: 114.18° γ: 90°
R-values:
R R work R free
0.173 0.171 0.215
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

Harnessing the Power of T Cells: The Promising Hope for a Universal Influenza Vaccine.
Clemens et al. (2018)
13 more

3 mentions without citation

Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation.
Wieczorek et al. (2017)
2 more