› 5hi2

X-ray diffraction
2.51Å resolution

BRAF Kinase domain b3aC loop deletion mutant in complex with sorafenib

Released:
DOI: 10.2210/pdb5hi2/pdb
PDB entry 5hi2 coloured by chain, front view.
PDB entry 5hi2 coloured by chain, side view.
PDB entry 5hi2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation Mechanism of Oncogenic Deletion Mutations in BRAF, EGFR, and HER2.
Foster SA, Whalen DM, Özen A, Wongchenko MJ, Yin J, Yen I, Schaefer G, Mayfield JD, Chmielecki J, Stephens PJ, Albacker LA, Yan Y, Song K, Hatzivassiliou G, Eigenbrot C, Yu C, Shaw AS, Manning G, Skelton NJ, Hymowitz SG, Malek S
Cancer Cell 29 477-493 (2016)
PMID: 26996308

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15056 (Residues: 444-723; Coverage: 36%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BAX 1 x BAX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 49.625Å b: 114.92Å c: 101.544Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.185 0.237
Expression system: Escherichia coli

Quick links

Citations

31 review citations

KRAS: The Critical Driver and Therapeutic Target for Pancreatic Cancer.
Waters et al. (2018)
30 more

6 mentions without citation

Challenges and Opportunities in the Crusade of BRAF Inhibitors: From 2002 to 2022.
Singh et al. (2023)
5 more