› 5hie

X-ray diffraction
3Å resolution

BRAF Kinase domain b3aC loop deletion mutant in complex with dabrafenib

Released:
DOI: 10.2210/pdb5hie/pdb
PDB entry 5hie coloured by chain, front view.
PDB entry 5hie coloured by chain, side view.
PDB entry 5hie coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation Mechanism of Oncogenic Deletion Mutations in BRAF, EGFR, and HER2.
Foster SA, Whalen DM, Özen A, Wongchenko MJ, Yin J, Yen I, Schaefer G, Mayfield JD, Chmielecki J, Stephens PJ, Albacker LA, Yan Y, Song K, Hatzivassiliou G, Eigenbrot C, Yu C, Shaw AS, Manning G, Skelton NJ, Hymowitz SG, Malek S
Cancer Cell 29 477-493 (2016)
PMID: 26996308

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 302 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P15056 (Residues: 432-726; Coverage: 38%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand P06 4 x P06
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 65.413Å b: 127.786Å c: 83.135Å
α: 90° β: 105.79° γ: 90°
R-values:
R R work R free
0.196 0.194 0.221
Expression system: Trichoplusia ni

Quick links

Citations

31 review citations

KRAS: The Critical Driver and Therapeutic Target for Pancreatic Cancer.
Waters et al. (2018)
30 more

1 mention without citation

Impact of the Protein Data Bank on antineoplastic approvals.
Westbrook et al. (2020)