› 5hkw

X-ray diffraction
2.25Å resolution

Crystal Structure of Apo c-Cbl TKBD Refined to 2.25 A Resolution

Released:
DOI: 10.2210/pdb5hkw/pdb
PDB entry 5hkw coloured by chain, front view.
PDB entry 5hkw coloured by chain, side view.
PDB entry 5hkw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Lovell S, Battaile KP, Mehzabeen N, Zhang N, Cooper A, Gao P, Perez RP

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase CBL Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 308 amino acids
Theoretical weight: 35.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22681 (Residues: 47-351; Coverage: 34%)
Gene names: CBL, CBL2, RNF55
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 159.116Å b: 106.523Å c: 84.984Å
α: 90° β: 92.13° γ: 90°
R-values:
R R work R free
0.182 0.18 0.234
Expression system: Escherichia coli BL21(DE3)

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