› 5hnb

X-ray diffraction
2.35Å resolution

CDK8-CYCC IN COMPLEX WITH [6-Hydroxy-3-(3-methyl-benzyl)-1H-indazol-5-yl]-((S)-3-hydroxy-pyrrolidin-1-yl)-methanone

Released:
DOI: 10.2210/pdb5hnb/pdb
PDB entry 5hnb coloured by chain, front view.
PDB entry 5hnb coloured by chain, side view.
PDB entry 5hnb coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Musil D, Blagg J, Schiemann K

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150300 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 8 Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 43.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49336 (Residues: 1-362; Coverage: 78%)
Gene name: CDK8
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-C Chain: B
Molecule details ›
Chain: B
Length: 266 amino acids
Theoretical weight: 31.54 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24863 (Residues: 1-264; Coverage: 93%)
Gene name: CCNC
Sequence domains:
Structure domains: Cyclin-like

Ligands and Environments

3 bound ligands:
Ligand 62M 1 x 62M
Ligand FMT 6 x FMT
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 71.232Å b: 71.439Å c: 171Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.252
Expression system: Spodoptera frugiperda

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