› 5hns

X-ray diffraction
2.45Å resolution

Structure of glycosylated NPC1 luminal domain C

Released:
DOI: 10.2210/pdb5hns/pdb
PDB entry 5hns coloured by chain, front view.
PDB entry 5hns coloured by chain, side view.
PDB entry 5hns coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of glycosylated NPC1 luminal domain C reveals insights into NPC2 and Ebola virus interactions.
Zhao Y, Ren J, Harlos K, Stuart DI
FEBS Lett 590 605-12 (2016)
PMID: 26846330

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
NPC intracellular cholesterol transporter 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 27.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O15118 (Residues: 387-618; Coverage: 19%)
Gene name: NPC1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand SCN 4 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 87.89Å b: 115.91Å c: 147.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.238
Expression system: Homo sapiens

Quick links

Citations

10 review citations

NPC intracellular cholesterol transporter 1 (NPC1)-mediated cholesterol export from lysosomes.
Pfeffer SR. (2019)
9 more

4 mentions without citation

Structural Insights into the Niemann-Pick C1 (NPC1)-Mediated Cholesterol Transfer and Ebola Infection.
Gong et al. (2016)
3 more