› 5hpb

X-ray diffraction
1.65Å resolution

human dihydrofolate reductase complex with NADPH and 5-methyl-6-(phenylthio-4'trifluoromethyl)thieno[2,3-d]pyrimidine-2,4-diamine

Released:
DOI: 10.2210/pdb5hpb/pdb
PDB entry 5hpb coloured by chain, front view.
PDB entry 5hpb coloured by chain, side view.
PDB entry 5hpb coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cody V, Ganjee A

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00374 (Residues: 2-187; Coverage: 100%)
Gene name: DHFR
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
2 bound ligands:
Ligand 63W 1 x 63W
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: R3
Unit cell:
a: 85.845Å b: 85.845Å c: 76.725Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.236 0.293
Expression system: Escherichia coli

Quick links