› 5hqi

X-ray diffraction
0.97Å resolution

Insulin with proline analog HzP at position B28 in the T2 state

Released:
DOI: 10.2210/pdb5hqi/pdb
PDB entry 5hqi coloured by chain, front view.
PDB entry 5hqi coloured by chain, side view.
PDB entry 5hqi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
4S-Hydroxylation of Insulin at ProB28 Accelerates Hexamer Dissociation and Delays Fibrillation.
Lieblich SA, Fang KY, Cahn JKB, Rawson J, LeBon J, Ku HT, Tirrell DA
J Am Chem Soc 139 8384-8387 (2017)
PMID: 28598606

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand HZP 1 x HZP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I213
Unit cell:
a: 78.234Å b: 78.234Å c: 78.234Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.158
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Future prospects for noncanonical amino acids in biological therapeutics.
Rezhdo et al. (2019)
11 other articles