› 5hsu

X-ray diffraction
1.46Å resolution

Fluorine substituted 5-methyl-6-(2',4'-difluoromethoxyphenythio)thieno[2,3-d]pyrimidine-2,4-diamine

Released:
DOI: 10.2210/pdb5hsu/pdb
PDB entry 5hsu coloured by chain, front view.
PDB entry 5hsu coloured by chain, side view.
PDB entry 5hsu coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Cody V

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00374 (Residues: 2-187; Coverage: 100%)
Gene name: DHFR
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand 63Y 1 x 63Y
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: R3
Unit cell:
a: 86.14Å b: 86.14Å c: 76.245Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.231 0.253
Expression system: Escherichia coli

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