› 5htk

X-ray diffraction
2.01Å resolution

Human Heart 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase (PFKFB2)

Released:
DOI: 10.2210/pdb5htk/pdb
PDB entry 5htk coloured by chain, front view.
PDB entry 5htk coloured by chain, side view.
PDB entry 5htk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) and the inhibitory influence of citrate on substrate binding.
Crochet RB, Kim JD, Lee H, Yim YS, Kim SG, Neau D, Lee YH
Proteins 85 117-124 (2017)
PMID: 27802586

Function and Biology Details

Reactions catalysed: 
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 58.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60825 (Residues: 1-505; Coverage: 100%)
Gene name: PFKFB2
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand FLC 2 x FLC
Ligand ATP 2 x ATP
Ligand MG 2 x MG
Ligand F6P 2 x F6P
Ligand EDO 3 x EDO
Ligand GOL 8 x GOL
Ligand MES 1 x MES
1 modified residue:
Ligand NEP 2 x NEP

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: P212121
Unit cell:
a: 106.523Å b: 113.946Å c: 133.154Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Fructose 2,6-Bisphosphate in Cancer Cell Metabolism.
Bartrons et al. (2018)
2 more

4 mentions without citation

Role of PFKFB3 and PFKFB4 in Cancer: Genetic Basis, Impact on Disease Development/Progression, and Potential as Therapeutic Targets.
Kotowski et al. (2021)
3 more