› 5hvj

X-ray diffraction
2.2Å resolution

Crystal structure of LIMK1 D460N mutant in complex with AMP-PNP

Released:
DOI: 10.2210/pdb5hvj/pdb
PDB entry 5hvj coloured by chain, front view.
PDB entry 5hvj coloured by chain, side view.
PDB entry 5hvj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Noncanonical Substrate Recognition of Cofilin/ADF Proteins by LIM Kinases.
Hamill S, Lou HJ, Turk BE, Boggon TJ
Mol Cell 62 397-408 (2016)
PMID: 27153537

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LIM domain kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 36.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53667 (Residues: 329-638; Coverage: 48%)
Gene names: LIMK, LIMK1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 54.987Å b: 59.573Å c: 98.276Å
α: 90° β: 101.28° γ: 90°
R-values:
R R work R free
0.208 0.206 0.252
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

Homing in: Mechanisms of Substrate Targeting by Protein Kinases.
Miller et al. (2018)
8 more

3 mentions without citation

Structural Aspects of LIMK Regulation and Pharmacology.
Chatterjee et al. (2022)
2 more