› 5hvk

X-ray diffraction
3.5Å resolution

Crystal structure of LIMK1 mutant D460N in complex with full-length cofilin-1

Released:
DOI: 10.2210/pdb5hvk/pdb
PDB entry 5hvk coloured by chain, front view.
PDB entry 5hvk coloured by chain, side view.
PDB entry 5hvk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Noncanonical Substrate Recognition of Cofilin/ADF Proteins by LIM Kinases.
Hamill S, Lou HJ, Turk BE, Boggon TJ
Mol Cell 62 397-408 (2016)
PMID: 27153537

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149995 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
LIM domain kinase 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 315 amino acids
Theoretical weight: 36.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53667 (Residues: 329-638; Coverage: 48%)
Gene names: LIMK, LIMK1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Cofilin-1 Chain: B
Molecule details ›
Chain: B
Length: 165 amino acids
Theoretical weight: 18.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23528 (Residues: 2-166; Coverage: 99%)
Gene names: CFL, CFL1
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin
Cofilin-1 Chain: D
Molecule details ›
Chain: D
Length: 165 amino acids
Theoretical weight: 18.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23528 (Residues: 2-166; Coverage: 99%)
Gene names: CFL, CFL1
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 81.116Å b: 102.283Å c: 141.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.273 0.31
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Cytoskeletal Remodeling in Cancer.
Aseervatham J. (2020)
7 more

8 mentions without citation

Homing in: Mechanisms of Substrate Targeting by Protein Kinases.
Miller et al. (2018)
7 more