5hxb

X-ray diffraction
3.6Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-147262 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Chains: A, X
Molecule details ›
Chains: A, X
Length: 199 amino acids
Theoretical weight: 22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15170 (Residues: 300-496; Coverage: 40%)
Gene names: ERF3A, GSPT1
Sequence domains:
Structure domains: Translation factors
DNA damage-binding protein 1 Chains: B, Y
Molecule details ›
Chains: B, Y
Length: 1140 amino acids
Theoretical weight: 127.1 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16531 (Residues: 1-1140; Coverage: 100%)
Gene names: DDB1, XAP1
Sequence domains:
Structure domains:
Protein cereblon Chains: C, Z
Molecule details ›
Chains: C, Z
Length: 406 amino acids
Theoretical weight: 46.6 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96SW2 (Residues: 40-442; Coverage: 91%)
Gene names: AD-006, CRBN
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P2
Unit cell:
a: 156.747Å b: 111.522Å c: 175.057Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.226 0.224 0.273
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda