› 5hy9

X-ray diffraction
2.7Å resolution

Glycosylated, disulfide-linked Knob-into-Hole Fc fragment

Released:
DOI: 10.2210/pdb5hy9/pdb
PDB entry 5hy9 coloured by chain, front view.
PDB entry 5hy9 coloured by chain, side view.
PDB entry 5hy9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural differences between glycosylated, disulfide-linked heterodimeric Knob-into-Hole Fc fragment and its homodimeric Knob-Knob and Hole-Hole side products.
Kuglstatter A, Stihle M, Neumann C, Müller C, Schaefer W, Klein C, Benz J
Protein Eng Des Sel 30 649-656 (2017)
PMID: 28985438

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134813 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 104-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 1 Chain: B
Molecule details ›
Chain: B
Length: 227 amino acids
Theoretical weight: 25.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 104-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, GAL, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6122
Unit cell:
a: 151.813Å b: 151.813Å c: 113.869Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.272
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Principles and Current Clinical Landscape of Multispecific Antibodies against Cancer.
Elshiaty et al. (2021)
4 more

5 mentions without citation

Immunoglobulin Fc Heterodimer Platform Technology: From Design to Applications in Therapeutic Antibodies and Proteins.
Ha et al. (2016)
4 more