› 5hys

X-ray diffraction
2.5Å resolution

Structure of IgE complexed with omalizumab

Released:
DOI: 10.2210/pdb5hys/pdb
PDB entry 5hys coloured by chain, front view.
PDB entry 5hys coloured by chain, side view.
PDB entry 5hys coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange.
Pennington LF, Tarchevskaya S, Brigger D, Sathiyamoorthy K, Graham MT, Nadeau KC, Eggel A, Jardetzky TS
Nat Commun 7 11610 (2016)
PMID: 27194387

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229772 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Epididymis luminal protein 214 Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 222 amino acids
Theoretical weight: 23.65 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Uncharacterized protein Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 218 amino acids
Theoretical weight: 23.92 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Immunoglobulin heavy constant epsilon Chains: G, I, J, K
Molecule details ›
Chains: G, I, J, K
Length: 230 amino acids
Theoretical weight: 25.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01854 (Residues: 209-427; Coverage: 40%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand SO4 48 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 100.103Å b: 107.143Å c: 151.043Å
α: 90° β: 95.18° γ: 90°
R-values:
R R work R free
0.221 0.221 0.239
Expression systems:
  • Cricetulus griseus
  • Spodoptera frugiperda

Quick links

Citations

26 review citations

Understanding Fc Receptor Involvement in Inflammatory Diseases: From Mechanisms to New Therapeutic Tools.
Ben Mkaddem et al. (2019)
25 more

5 mentions without citation

The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab.
Gasser et al. (2020)
4 more