› 5hyt

X-ray diffraction
2.54Å resolution

Structure of human C4b-binidng protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M22 protein

Released:
DOI: 10.2210/pdb5hyt/pdb
PDB entry 5hyt coloured by chain, front view.
PDB entry 5hyt coloured by chain, side view.
PDB entry 5hyt coloured by chain, top view.
Source organisms:
Primary publication:
Conserved patterns hidden within group A Streptococcus M protein hypervariability recognize human C4b-binding protein.
Buffalo CZ, Bahn-Suh AJ, Hirakis SP, Biswas T, Amaro RE, Nizet V, Ghosh P
Nat Microbiol 1 16155 (2016)
PMID: 27595425

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137315 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 83 amino acids
Theoretical weight: 9.87 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54901 (Residues: 48-126; Coverage: 22%)
Gene name: sir22
C4b-binding protein alpha chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 128 amino acids
Theoretical weight: 14.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04003 (Residues: 49-172; Coverage: 23%)
Gene names: C4BP, C4BPA
Sequence domains: Sushi repeat (SCR repeat)
Structure domains: Complement Module, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 68.079Å b: 80.348Å c: 152.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.213 0.274
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Group A streptococcal M-like proteins: From pathogenesis to vaccine potential.
Frost et al. (2018)
5 more

1 mention without citation

Structure-based group A streptococcal vaccine design: Helical wheel homology predicts antibody cross-reactivity among streptococcal M protein-derived peptides.
Aranha et al. (2020)