› 5i7m

X-ray diffraction
1.93Å resolution

Crystal structure of Y345F mutant of human primase p58 iron-sulfur cluster domain

Released:
DOI: 10.2210/pdb5i7m/pdb
PDB entry 5i7m coloured by chain, front view.
PDB entry 5i7m coloured by chain, side view.
PDB entry 5i7m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport.
O'Brien E, Holt ME, Thompson MK, Salay LE, Ehlinger AC, Chazin WJ, Barton JK
Science 355 (2017)
PMID: 28232525

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase large subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49643 (Residues: 272-457; Coverage: 37%)
Gene names: PRIM2, PRIM2A
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit

Ligands and Environments

2 bound ligands:
Ligand SF4 2 x SF4
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 109.963Å b: 52.624Å c: 89.195Å
α: 90° β: 115.27° γ: 90°
R-values:
R R work R free
0.213 0.211 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

The elemental role of iron in DNA synthesis and repair.
Puig et al. (2017)
10 more

1 mention without citation

Comment on "The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport".
Baranovskiy et al. (2017)