› 5ifs

X-ray diffraction
2.46Å resolution

Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in ASPL that disrupts functional p97 hexamers and induces cell death

Released:
DOI: 10.2210/pdb5ifs/pdb
PDB entry 5ifs coloured by chain, front view.
PDB entry 5ifs coloured by chain, side view.
PDB entry 5ifs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers.
Arumughan A, Roske Y, Barth C, Forero LL, Bravo-Rodriguez K, Redel A, Kostova S, McShane E, Opitz R, Faelber K, Rau K, Mielke T, Daumke O, Selbach M, Sanchez-Garcia E, Rocks O, Panáková D, Heinemann U, Wanker EE
Nat Commun 7 13047 (2016)
PMID: 27762274

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157180 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tether containing UBX domain for GLUT4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 237 amino acids
Theoretical weight: 26.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BZE9 (Residues: 317-553; Coverage: 43%)
Gene names: ASPL, ASPSCR1, RCC17, TUG, UBXD9, UBXN9
Sequence domains: UBX domain
Transitional endoplasmic reticulum ATPase Chains: B, D
Molecule details ›
Chains: B, D
Length: 481 amino acids
Theoretical weight: 53.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55072 (Residues: 1-481; Coverage: 60%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand SO4 1 x SO4
Ligand EDO 3 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 69.844Å b: 132.914Å c: 96.121Å
α: 90° β: 110.15° γ: 90°
R-values:
R R work R free
0.204 0.201 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Interplay of Cofactor Interactions and Post-translational Modifications in the Regulation of the AAA+ ATPase p97.
Hänzelmann et al. (2017)
4 more

10 mentions without citation

The functional importance of VCP to maintaining cellular protein homeostasis.
Ahlstedt et al. (2022)
9 more