› 5ifw

X-ray diffraction
3.4Å resolution

Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in ASPL that disrupts functional p97 hexamers and induces cell death

Released:
DOI: 10.2210/pdb5ifw/pdb
PDB entry 5ifw coloured by chain, front view.
PDB entry 5ifw coloured by chain, side view.
PDB entry 5ifw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers.
Arumughan A, Roske Y, Barth C, Forero LL, Bravo-Rodriguez K, Redel A, Kostova S, McShane E, Opitz R, Faelber K, Rau K, Mielke T, Daumke O, Selbach M, Sanchez-Garcia E, Rocks O, Panáková D, Heinemann U, Wanker EE
Nat Commun 7 13047 (2016)
PMID: 27762274

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157180 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tether containing UBX domain for GLUT4 Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BZE9 (Residues: 317-504; Coverage: 34%)
Gene names: ASPL, ASPSCR1, RCC17, TUG, UBXD9, UBXN9
Sequence domains: UBX domain
Transitional endoplasmic reticulum ATPase Chain: B
Molecule details ›
Chain: B
Length: 807 amino acids
Theoretical weight: 89.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55072 (Residues: 2-806; Coverage: 100%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C222
Unit cell:
a: 143.344Å b: 208.361Å c: 99.129Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The AAA+ ATPase p97, a cellular multitool.
Stach et al. (2017)
6 more

4 mentions without citation

The Interplay of Cofactor Interactions and Post-translational Modifications in the Regulation of the AAA+ ATPase p97.
Hänzelmann et al. (2017)
3 more