› 5ii0

X-ray diffraction
2.1Å resolution

Crystal structure of the human calcitonin receptor ectodomain in complex with a truncated salmon calcitonin analogue

Released:
DOI: 10.2210/pdb5ii0/pdb
PDB entry 5ii0 coloured by chain, front view.
PDB entry 5ii0 coloured by chain, side view.
PDB entry 5ii0 coloured by chain, top view.
Source organisms:
Primary publication:
Type II Turn of Receptor-bound Salmon Calcitonin Revealed by X-ray Crystallography.
Johansson E, Hansen JL, Hansen AM, Shaw AC, Becker P, Schäffer L, Reedtz-Runge S
J Biol Chem 291 13689-98 (2016)
PMID: 27189946

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151858 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcitonin receptor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 122 amino acids
Theoretical weight: 14.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30988 (Residues: 25-144; Coverage: 27%)
Gene name: CALCR
Sequence domains: Hormone receptor domain
Structure domains: GPCR, family 2, extracellular hormone receptor domain
Calcitonin peptide-like domain-containing protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 25 amino acids
Theoretical weight: 2.79 KDa
Source organism: Oncorhynchus gorbuscha
Expression system: Not provided
UniProt:
  • Canonical: Q91970 (Residues: 13-37; Coverage: 42%)
Gene name: CT
Sequence domains: Calcitonin / CGRP / IAPP family

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand URE 1 x URE
1 modified residue:
Ligand LPD 3 x LPD

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002+
Spacegroup: C2
Unit cell:
a: 96.558Å b: 113.165Å c: 55.424Å
α: 90° β: 114.79° γ: 90°
R-values:
R R work R free
0.172 0.17 0.206
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Advances in therapeutic peptides targeting G protein-coupled receptors.
Davenport et al. (2020)
2 more

10 mentions without citation

Update on the pharmacology of calcitonin/CGRP family of peptides: IUPHAR Review 25.
Hay et al. (2018)
9 more