› 5im0

X-ray diffraction
1.7Å resolution

Crystal structure of the RNA recognition motif of mRNA decay regulator AUF1

Released:
DOI: 10.2210/pdb5im0/pdb
PDB entry 5im0 coloured by chain, front view.
PDB entry 5im0 coloured by chain, side view.
PDB entry 5im0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of the N-Terminal RNA Recognition Motif of mRNA Decay Regulator AUF1.
Choi YJ, Yoon JH, Chang JH
Biomed Res Int 2016 3286191 (2016)
PMID: 27437398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171906 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heterogeneous nuclear ribonucleoprotein D0 Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.18 KDa
Source organism: Homo sapiens
Expression system: Enterobacteria phage L1
UniProt:
  • Canonical: Q14103 (Residues: 93-175; Coverage: 23%)
Gene names: AUF1, HNRNPD, HNRPD
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Heterogeneous nuclear ribonucleoprotein D0 Chain: B
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.48 KDa
Source organism: Homo sapiens
Expression system: Enterobacteria phage L1
UniProt:
  • Canonical: Q14103 (Residues: 96-175; Coverage: 23%)
Gene names: AUF1, HNRNPD, HNRPD
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 39.068Å b: 39.411Å c: 93.252Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.204
Expression system: Enterobacteria phage L1

Quick links

Citations

1 review citation

Heterogeneous Nuclear Ribonucleoproteins Involved in the Functioning of Telomeres in Malignant Cells.
Shishkin et al. (2019)
4 other articles

3 mentions without citation

Characterization of epitranscriptome reader proteins experimentally and in silico: Current knowledge and future perspectives beyond the YTH domain.
Miller et al. (2023)
2 more