› 5iok

X-ray diffraction
2.22Å resolution

Crystal structure of Taf14 YEATS domain in complex with histone H3K9cr

Released:
DOI: 10.2210/pdb5iok/pdb
PDB entry 5iok coloured by chain, front view.
PDB entry 5iok coloured by chain, side view.
PDB entry 5iok coloured by chain, top view.
Source organisms:
Primary publication:
The Taf14 YEATS domain is a reader of histone crotonylation.
Andrews FH, Shinsky SA, Shanle EK, Bridgers JB, Gest A, Tsun IK, Krajewski K, Shi X, Strahl BD, Kutateladze TG
Nat Chem Biol 12 396-8 (2016)
PMID: 27089029

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152814 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription initiation factor TFIID subunit 14 Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P35189 (Residues: 1-137; Coverage: 56%)
Gene names: ANC1, CST10, SWP29, TAF14, TAF30, TFG3, YPL129W
Sequence domains: YEATS family
Structure domains: YEATS domain
Histone H3 Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 886 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P61830 (Residues: 6-12; Coverage: 5%)
Gene names: HHT1, HHT2, N2749, SIN2, YBR010W, YBR0201, YNL031C

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
1 modified residue:
Ligand KCR 1 x KCR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P65
Unit cell:
a: 113.095Å b: 113.095Å c: 26.176Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

41 review citations

Functions of bromodomain-containing proteins and their roles in homeostasis and cancer.
Fujisawa et al. (2017)
40 more

7 mentions without citation

Metabolic regulation of gene expression through histone acylations.
Sabari et al. (2017)
6 more