5iro

X-ray diffraction
2.64Å resolution

Crystal structure of a complex between the Human adenovirus type 4 E3-19K protein and MHC class molecule HLA-A2/TAX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-137668 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, E, I, M, Q, U
Molecule details ›
Chains: A, E, I, M, Q, U
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Protein Tax-1 Chains: B, F, J, N, R, V
Molecule details ›
Chains: B, F, J, N, R, V
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell leukemia virus type I
Expression system: Not provided
UniProt:
  • Canonical: P14079 (Residues: 11-19; Coverage: 3%)
Gene name: tax
Beta-2-microglobulin Chains: C, G, K, O, S, W
Molecule details ›
Chains: C, G, K, O, S, W
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Early E3 18.5 kDa glycoprotein Chains: D, H, L, P, T, X
Molecule details ›
Chains: D, H, L, P, T, X
Length: 108 amino acids
Theoretical weight: 12.39 KDa
Source organism: Human adenovirus E4
Expression system: Human adenovirus E4
UniProt:
  • Canonical: Q8BEL5 (Residues: 24-131; Coverage: 62%)
Gene names: E3, HAdVE_gp12
Sequence domains: Adenovirus GP19K
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3
Unit cell:
a: 165.727Å b: 165.727Å c: 122.858Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.259 0.257 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Komagataella pastoris
  • Human adenovirus E4