› 5irs

X-ray diffraction
1.8Å resolution

crystal structure of the proteasomal Rpn13 PRU-domain

Released:
DOI: 10.2210/pdb5irs/pdb
PDB entry 5irs coloured by chain, front view.
PDB entry 5irs coloured by chain, side view.
PDB entry 5irs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.
Chen X, Randles L, Shi K, Tarasov SG, Aihara H, Walters KJ
Structure 24 1257-1270 (2016)
PMID: 27396824

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteasomal ubiquitin receptor ADRM1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16186 (Residues: 2-150; Coverage: 37%)
Gene names: ADRM1, GP110
Sequence domains: Proteasome complex subunit Rpn13, Pru domain
Structure domains: Proteasomal ubiquitin receptor Rpn13/ADRM1

Ligands and Environments

1 bound ligand:
Ligand DTT 2 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 42.555Å b: 56.476Å c: 64.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.167 0.209
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
15 more

7 mentions without citation

Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets.
Lu et al. (2017)
6 more