5isz

X-ray diffraction
2.06Å resolution

Crystal structure of LS01-TCR/M1-HLA-A*02 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-229821 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 22-119; Coverage: 99%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Matrix protein 1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 966 Da
Source organism: Influenza A virus
Expression system: Not provided
UniProt:
  • Canonical: P03485 (Residues: 58-66; Coverage: 4%)
Gene name: M
TCRalpha chain Chain: D
Molecule details ›
Chain: D
Length: 200 amino acids
Theoretical weight: 22.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
TCRbeta chain Chain: E
Molecule details ›
Chain: E
Length: 241 amino acids
Theoretical weight: 27.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 63.81Å b: 75.46Å c: 121.2Å
α: 90° β: 98.01° γ: 90°
R-values:
R R work R free
0.185 0.184 0.2
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Escherichia coli