› 5iza

X-ray diffraction
1.5Å resolution

Protein-protein interaction

Released:
DOI: 10.2210/pdb5iza/pdb
PDB entry 5iza coloured by chain, front view.
PDB entry 5iza coloured by chain, side view.
PDB entry 5iza coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration.
Jiang H, Deng R, Yang X, Shang J, Lu S, Zhao Y, Song K, Liu X, Zhang Q, Chen Y, Chinn YE, Wu G, Li J, Chen G, Yu J, Zhang J
Nat Chem Biol 13 994-1001 (2017)
PMID: 28759015

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150346 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Adenomatous polyposis coli protein Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25054 (Residues: 407-751; Coverage: 12%)
Gene names: APC, DP2.5
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
ACE-GLY-GLY-GLU-ALA-LEU-ALA-TRP-NH2 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 727 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 52.465Å b: 66.436Å c: 82.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.121 0.159
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Inhibitors of protein-protein interactions (PPIs): an analysis of scaffold choices and buried surface area.
Ran et al. (2018)
4 more

3 mentions without citation

Advances and Insights of APC-Asef Inhibitors for Metastatic Colorectal Cancer Therapy.
Yang et al. (2021)
2 more