5j12

X-ray diffraction
3.55Å resolution

Structure of human TSLP:TSLPR in complex with mouse IL-7Ralpha

Released:
Source organisms:
Entry authors: Verstraete K, Savvides SN

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-147830 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thymic stromal lymphopoietin Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.52 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q969D9 (Residues: 1-159; Coverage: 96%)
Gene name: TSLP
Sequence domains: Thymic stromal lymphopoietin
Structure domains: Thymic stromal lymphopoietin
Interleukin-7 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 223 amino acids
Theoretical weight: 25.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16872 (Residues: 21-239; Coverage: 50%)
Gene name: Il7r
Sequence domains:
Structure domains:
Cytokine receptor-like factor 2 Chain: C
Molecule details ›
Chain: C
Length: 230 amino acids
Theoretical weight: 26.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9HC73 (Residues: 1-221; Coverage: 57%)
Gene names: CRL2, CRLF2, ILXR, TSLPR
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6522
Unit cell:
a: 125.442Å b: 125.442Å c: 194.573Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.225
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)