› 5j1g

X-ray diffraction
1.8Å resolution

Structure of the spectrin repeats 7 and 8 of the plakin domain of plectin

Released:
DOI: 10.2210/pdb5j1g/pdb
PDB entry 5j1g coloured by chain, front view.
PDB entry 5j1g coloured by chain, side view.
PDB entry 5j1g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.
Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM
J Biol Chem 291 18643-62 (2016)
PMID: 27413182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 26.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15149 (Residues: 1114-1343; Coverage: 5%)
Gene names: PLEC, PLEC1
Sequence domains: Spectrin repeat
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

2 bound ligands:
Ligand 1PE 2 x 1PE
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 45.68Å b: 115.94Å c: 64.8Å
α: 90° β: 97.56° γ: 90°
R-values:
R R work R free
0.189 0.187 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The spectraplakins of Caenorhabditis elegans: Cytoskeletal crosslinkers and beyond.
Fu et al. (2017)
5 more

1 mention without citation

The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.
Ortega et al. (2016)