› 5j9s

X-ray diffraction
2.7Å resolution

ENL YEATS in complex with histone H3 acetylation at K27

Released:
DOI: 10.2210/pdb5j9s/pdb
PDB entry 5j9s coloured by chain, front view.
PDB entry 5j9s coloured by chain, side view.
PDB entry 5j9s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
ENL links histone acetylation to oncogenic gene expression in acute myeloid leukaemia.
Wan L, Wen H, Li Y, Lyu J, Xi Y, Hoshii T, Joseph JK, Wang X, Loh YE, Erb MA, Souza AL, Bradner JE, Shen L, Li W, Li H, Allis CD, Armstrong SA, Shi X
Nature 543 265-269 (2017)
PMID: 28241141

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159401 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein ENL Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 18.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q03111 (Residues: 1-148; Coverage: 27%)
Gene names: ENL, LTG19, MLLT1, YEATS1
Sequence domains: YEATS family
Structure domains: YEATS domain
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 16-40; Coverage: 18%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3121
Unit cell:
a: 104.979Å b: 104.979Å c: 45.128Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.21
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

40 review citations

Born to run: control of transcription elongation by RNA polymerase II.
Chen et al. (2018)
39 more

9 mentions without citation

Structure, function and inhibition of critical protein-protein interactions involving mixed lineage leukemia 1 and its fusion oncoproteins.
Li et al. (2021)
8 more