› 5jcy

X-ray diffraction
1.8Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186753 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-Va Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 45.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y4I1 (Residues: 1464-1855; Coverage: 21%)
Gene names: MYH12, MYO5A
Sequence domains: DIL domain
Protein spire homolog 2 Chain: B
Molecule details ›
Chain: B
Length: 27 amino acids
Theoretical weight: 3.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8WWL2 (Residues: 401-427; Coverage: 4%)
Gene names: KIAA1832, SPIR2, SPIRE2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 99.61Å b: 41.22Å c: 108.22Å
α: 90° β: 115.89° γ: 90°
R-values:
R R work R free
0.153 0.151 0.191
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Actin assembly mechanisms at a glance.
Rottner et al. (2017)
9 more

4 mentions without citation

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.
Pylypenko et al. (2016)
3 more