5jhd

X-ray diffraction
2.46Å resolution

Crystal structure of LS10-TCR/M1-HLA-A*02 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-229822 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 276 amino acids
Theoretical weight: 31.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Matrix protein 1 Chains: C, H
Molecule details ›
Chains: C, H
Length: 9 amino acids
Theoretical weight: 966 Da
Source organism: Influenza A virus
Expression system: Not provided
UniProt:
  • Canonical: P03485 (Residues: 58-66; Coverage: 4%)
Gene name: M
TCRalpha chain Chains: D, I
Molecule details ›
Chains: D, I
Length: 213 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
TCRbeta chain Chains: E, J
Molecule details ›
Chains: E, J
Length: 242 amino acids
Theoretical weight: 27.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 49.71Å b: 101.84Å c: 113.55Å
α: 99.38° β: 92.6° γ: 103.45°
R-values:
R R work R free
0.205 0.204 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided