› 5jjd

X-ray diffraction
2.4Å resolution

crystal structure of the ceramide transfer protein PH and START domain complex

Released:
DOI: 10.2210/pdb5jjd/pdb
PDB entry 5jjd coloured by chain, front view.
PDB entry 5jjd coloured by chain, side view.
PDB entry 5jjd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Interaction between the PH and START domains of ceramide transfer protein competes with phosphatidylinositol 4-phosphate binding by the PH domain.
Prashek J, Bouyain S, Fu M, Li Y, Berkes D, Yao X
J Biol Chem 292 14217-14228 (2017)
PMID: 28652409

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195281 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ceramide transfer protein Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 12.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5P4 (Residues: 20-122; Coverage: 17%)
Gene names: CERT, CERT1, COL4A3BP, STARD11
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Ceramide transfer protein Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5P4 (Residues: 385-624; Coverage: 39%)
Gene names: CERT, CERT1, COL4A3BP, STARD11
Sequence domains: START domain
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

2 bound ligands:
Ligand PEG 3 x PEG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 59.761Å b: 60.914Å c: 95.804Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.245
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Lipid transfer proteins: the lipid commute via shuttles, bridges and tubes.
Wong et al. (2019)
7 more

4 mentions without citation

Interaction between the PH and START domains of ceramide transfer protein competes with phosphatidylinositol 4-phosphate binding by the PH domain.
Prashek et al. (2017)
3 more