Function and Biology Details
Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144089 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Botulinum neurotoxin A heavy chain
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 49.88 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 428 amino acids
Theoretical weight: 49.88 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
- Canonical:
P0DPI1 (Residues: 872-1296; Coverage: 33%)
Sequence domains:
- Clostridium neurotoxin, N-terminal receptor binding
- Clostridium neurotoxin, C-terminal receptor binding
Synaptic vesicle glycoprotein 2C
Molecule details ›
Chains: C, D
Length: 117 amino acids
Theoretical weight: 13.59 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector pCBio
UniProt:
Sequence domains: Pentapeptide repeats (9 copies)
Structure domains: E3 ubiquitin-protein ligase SopA
Length: 117 amino acids
Theoretical weight: 13.59 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector pCBio
UniProt:
- Canonical: Q496J9 (Residues: 473-567; Coverage: 13%)
Sequence domains: Pentapeptide repeats (9 copies)
Structure domains: E3 ubiquitin-protein ligase SopA
Ligands and Environments
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 24-ID-E
Spacegroup:
C2
Expression systems:
- Escherichia coli
- Mammalian expression vector pCBio
