› 5jmy

X-ray diffraction
2Å resolution

NEPRILYSIN COMPLEXED WITH LBQ657

Released:
DOI: 10.2210/pdb5jmy/pdb
PDB entry 5jmy coloured by chain, front view.
PDB entry 5jmy coloured by chain, side view.
PDB entry 5jmy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of neprilysin in complex with the active metabolite of sacubitril.
Schiering N, D'Arcy A, Villard F, Ramage P, Logel C, Cumin F, Ksander GM, Wiesmann C, Karki RG, Mogi M
Sci Rep 6 27909 (2016)
PMID: 27302413

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neprilysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 698 amino acids
Theoretical weight: 79.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08473 (Residues: 53-750; Coverage: 93%)
Gene names: EPN, MME
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 6 x NAG
Ligand ZN 2 x ZN
Ligand 6LD 2 x 6LD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 59.737Å b: 109.139Å c: 248.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.228
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Novel Therapeutic Approaches Targeting the Renin-Angiotensin System and Associated Peptides in Hypertension and Heart Failure.
Arendse et al. (2019)
3 more

9 mentions without citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
8 more