5jnx

Electron Microscopy
6.56Å resolution

The 6.6 A cryo-EM structure of the full-length human NPC1 in complex with the cleaved glycoprotein of Ebola virus

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo trimer
PDBe Complex ID:
PDB-CPX-127300 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
NPC intracellular cholesterol transporter 1 Chain: A
Molecule details ›
Chain: A
Length: 1278 amino acids
Theoretical weight: 142.27 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O15118 (Residues: 1-1278; Coverage: 100%)
Gene name: NPC1
Sequence domains:
GP1 Chains: C, E, G
Molecule details ›
Chains: C, E, G
Length: 158 amino acids
Theoretical weight: 17.19 KDa
Source organism: Ebola virus - Zaire (1995)
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P87666 (Residues: 32-188; Coverage: 24%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
Shed GP Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 130 amino acids
Theoretical weight: 14.88 KDa
Source organism: Ebola virus - Zaire (1995)
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P87666 (Residues: 509-632; Coverage: 19%)
Gene name: GP

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.56Å
Relevant EMDB volumes: EMD-8169
Expression systems:
  • Homo sapiens
  • Insect cell expression vector pTIE1