› 5joe

X-ray diffraction
2Å resolution

Crystal structure of I81 from titin

Released:
DOI: 10.2210/pdb5joe/pdb
PDB entry 5joe coloured by chain, front view.
PDB entry 5joe coloured by chain, side view.
PDB entry 5joe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex.
Zhou T, Fleming JR, Franke B, Bogomolovas J, Barsukov I, Rigden DJ, Labeit S, Mayans O
FEBS Lett 590 3098-110 (2016)
PMID: 27531639

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Titin Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WZ42 (Residues: 9582-9671; Coverage: 0%)
Gene name: TTN
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand IPA 1 x IPA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6522
Unit cell:
a: 115.31Å b: 115.31Å c: 52.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.218
Expression system: Escherichia coli

Quick links

Citations

4 review citations

N2A Titin: Signaling Hub and Mechanical Switch in Skeletal Muscle.
Nishikawa et al. (2020)
3 more

4 mentions without citation

Molecular Characterisation of Titin N2A and Its Binding of CARP Reveals a Titin/Actin Cross-linking Mechanism.
Zhou et al. (2021)
3 more