› 5jpx

Solution NMR

Solution structure of the TRIM21 B-box2 (B2)

Released:
DOI: 10.2210/pdb5jpx/pdb
PDB entry 5jpx coloured by chain, ensemble of 20 models, front view.
PDB entry 5jpx coloured by chain, ensemble of 20 models, side view.
PDB entry 5jpx coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution NMR structure of the TRIM21 B-box2 and identification of residues involved in its interaction with the RING domain.
Wallenhammar A, Anandapadamanaban M, Lemak A, Mirabello C, Lundström P, Wallner B, Sunnerhagen M
PLoS One 12 e0181551 (2017)
PMID: 28753623

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase TRIM21 Chain: A
Molecule details ›
Chain: A
Length: 45 amino acids
Theoretical weight: 5.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19474 (Residues: 86-130; Coverage: 10%)
Gene names: RNF81, RO52, SSA1, TRIM21
Sequence domains: B-box zinc finger
Structure domains: Classic Zinc Finger

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

1 review citation

TRIM21/Ro52 - Roles in Innate Immunity and Autoimmune Disease.
Jones et al. (2021)
1 other article

3 mentions without citation

To Ubiquitinate or Not to Ubiquitinate: TRIM17 in Cell Life and Death.
Basu-Shrivastava et al. (2021)
2 more