› 5jq1

X-ray diffraction
1.83Å resolution

Efficient targeting of the asialoglycoprotein receptor by polyvalent display of a compact galactosamine mimic

Released:
DOI: 10.2210/pdb5jq1/pdb
PDB entry 5jq1 coloured by chain, front view.
PDB entry 5jq1 coloured by chain, side view.
PDB entry 5jq1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Efficient Liver Targeting by Polyvalent Display of a Compact Ligand for the Asialoglycoprotein Receptor.
Sanhueza CA, Baksh MM, Thuma B, Roy MD, Dutta S, Préville C, Chrunyk BA, Beaumont K, Dullea R, Ammirati M, Liu S, Gebhard D, Finley JE, Salatto CT, King-Ahmad A, Stock I, Atkinson K, Reidich B, Lin W, Kumar R, Tu M, Menhaji-Klotz E, Price DA, Liras S, Finn MG, Mascitti V
J Am Chem Soc 139 3528-3536 (2017)
PMID: 28230359

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Asialoglycoprotein receptor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 17.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: P07306 (Residues: 148-291; Coverage: 50%)
Gene names: ASGR1, CLEC4H1
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand ZPF 2 x ZPF
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 41.08Å b: 59.49Å c: 68.65Å
α: 90° β: 93.18° γ: 90°
R-values:
R R work R free
0.189 0.187 0.222
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

3 review citations

Therapeutic siRNA: state of the art.
Hu et al. (2020)
2 more

3 mentions without citation

Glycomimetics for the inhibition and modulation of lectins.
Leusmann et al. (2023)
2 more