› 5jqa

X-ray diffraction
1.8Å resolution

CaM:RM20 complex

Released:
DOI: 10.2210/pdb5jqa/pdb
PDB entry 5jqa coloured by chain, front view.
PDB entry 5jqa coloured by chain, side view.
PDB entry 5jqa coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tokars VL, Minasov G, Anderson WF, Watterson DM

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143951 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Myosin light chain kinase, smooth muscle, deglutamylated form Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15746 (Residues: 1742-1761; Coverage: 1%)
Gene names: MLCK, MLCK1, MYLK, MYLK1

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 29.016Å b: 57.048Å c: 45.068Å
α: 90° β: 98.17° γ: 90°
R-values:
R R work R free
0.155 0.153 0.188
Expression systems:
  • Escherichia coli
  • Not provided

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