› 5jqh

X-ray diffraction
3.2Å resolution

Structure of beta2 adrenoceptor bound to carazolol and inactive-state stabilizing nanobody, Nb60

Released:
DOI: 10.2210/pdb5jqh/pdb
PDB entry 5jqh coloured by chain, front view.
PDB entry 5jqh coloured by chain, side view.
PDB entry 5jqh coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation.
Staus DP, Strachan RT, Manglik A, Pani B, Kahsai AW, Kim TH, Wingler LM, Ahn S, Chatterjee A, Masoudi A, Kruse AC, Pardon E, Steyaert J, Weis WI, Prosser RS, Kobilka BK, Costa T, Lefkowitz RJ
Nature 535 448-52 (2016)
PMID: 27409812

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229759 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-2 adrenergic receptor; Endolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 471 amino acids
Theoretical weight: 53.7 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
  • Canonical: P07550 (Residues: 30-348; Coverage: 71%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:
Nanobody60, Nb60 Chains: C, D
Molecule details ›
Chains: C, D
Length: 125 amino acids
Theoretical weight: 13.53 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CAU 2 x CAU
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 43.924Å b: 164.486Å c: 218.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.246 0.29
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

49 review citations

The Molecular Basis of G Protein-Coupled Receptor Activation.
Weis et al. (2018)
48 more

26 mentions without citation

How Ligands Illuminate GPCR Molecular Pharmacology.
Wacker et al. (2017)
25 more