› 5jrt

X-ray diffraction
1.53Å resolution

Crystal structure of the human Tankyrase 2 (TNKS2) SAM domain (DH902/924RE)

Released:
DOI: 10.2210/pdb5jrt/pdb
PDB entry 5jrt coloured by chain, front view.
PDB entry 5jrt coloured by chain, side view.
PDB entry 5jrt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tankyrase Requires SAM Domain-Dependent Polymerization to Support Wnt-β-Catenin Signaling.
Mariotti L, Templeton CM, Ranes M, Paracuellos P, Cronin N, Beuron F, Morris E, Guettler S
Mol Cell 63 498-513 (2016)
PMID: 27494558

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase tankyrase-2 Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 8.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2K2 (Residues: 867-940; Coverage: 6%)
Gene names: PARP5B, TANK2, TNKL, TNKS2
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P65
Unit cell:
a: 56.63Å b: 56.63Å c: 46.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.235
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Poly(ADP-ribose) polymerase enzymes and the maintenance of genome integrity.
Eisemann et al. (2020)
17 more

2 mentions without citation

Tankyrase Requires SAM Domain-Dependent Polymerization to Support Wnt-β-Catenin Signaling.
Mariotti et al. (2016)
1 more