› 5juw

X-ray diffraction
2.28Å resolution

complex of Dot1l with SS148

Released:
DOI: 10.2210/pdb5juw/pdb
PDB entry 5juw coloured by chain, front view.
PDB entry 5juw coloured by chain, side view.
PDB entry 5juw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Yu W, Tempel W, Li Y, Spurr SS, Bayle ED, Fish PV, Schapira M, Arrowsmith CH, Edwards AM, Bountra C, Weigelt J, Brown PJ, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(79) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(79)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase, H3 lysine-79 specific Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 47.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TEK3 (Residues: 1-420; Coverage: 27%)
Gene names: DOT1L, KIAA1814, KMT4
Sequence domains: Histone methylation protein DOT1
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand UNX 22 x UNX
Ligand 6NR 1 x 6NR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 153.258Å b: 153.258Å c: 50.723Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.189 0.215
Expression system: Escherichia coli

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