› 5jvs

X-ray diffraction
2.25Å resolution

The neck-linker + DAL and alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1

Released:
DOI: 10.2210/pdb5jvs/pdb
PDB entry 5jvs coloured by chain, front view.
PDB entry 5jvs coloured by chain, side view.
PDB entry 5jvs coloured by chain, top view.
Source organisms:
Primary publication:
Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips RK, Peter LG, Gilbert SP, Rayment I
J Biol Chem 291 20372-86 (2016)
PMID: 27462072

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin heavy chain; Microtubule-associated protein RP/EB family member 1 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.37 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17210 (Residues: 334-344, 345-367; Coverage: 4%)
  • Canonical: Q15691 (Residues: 209-257; Coverage: 18%)
Gene names: CG7765, Khc, MAPRE1, kin
Sequence domains: EB1-like C-terminal motif

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4122
Unit cell:
a: 40.016Å b: 40.016Å c: 185.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Intraflagellar transport trains and motors: Insights from structure.
Webb et al. (2020)
2 more

1 mention without citation

Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips et al. (2016)