› 5jvu

X-ray diffraction
1.95Å resolution

The neck-linker and alpha 7 helix of Drosophila melanogaster kinesin-1 fused to EB1

Released:
DOI: 10.2210/pdb5jvu/pdb
PDB entry 5jvu coloured by chain, front view.
PDB entry 5jvu coloured by chain, side view.
PDB entry 5jvu coloured by chain, top view.
Source organisms:
Primary publication:
Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips RK, Peter LG, Gilbert SP, Rayment I
J Biol Chem 291 20372-86 (2016)
PMID: 27462072

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin heavy chain; Microtubule-associated protein RP/EB family member 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 10.07 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17210 (Residues: 334-367; Coverage: 4%)
  • Canonical: Q15691 (Residues: 209-257; Coverage: 18%)
Gene names: CG7765, Khc, MAPRE1, kin
Sequence domains: EB1-like C-terminal motif

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 46.973Å b: 37.783Å c: 52.706Å
α: 90° β: 114.96° γ: 90°
R-values:
R R work R free
0.2 0.198 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Intraflagellar transport trains and motors: Insights from structure.
Webb et al. (2020)
2 more

1 mention without citation

Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips et al. (2016)