› 5jw9

X-ray diffraction
2Å resolution

The Crystal Structure of ELL2 Oclludin Domain and AFF4 peptide

Released:
DOI: 10.2210/pdb5jw9/pdb
PDB entry 5jw9 coloured by chain, front view.
PDB entry 5jw9 coloured by chain, side view.
PDB entry 5jw9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription.
Qi S, Li Z, Schulze-Gahmen U, Stjepanovic G, Zhou Q, Hurley JH
Nat Commun 8 14076 (2017)
PMID: 28134250

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126299 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AF4/FMR2 family member 4 Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 6.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHB7 (Residues: 301-351; Coverage: 4%)
Gene names: AF5Q31, AFF4, HSPC092, MCEF
RNA polymerase II elongation factor ELL2 Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 14.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00472 (Residues: 519-640; Coverage: 19%)
Gene name: ELL2
Sequence domains: Occludin homology domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 52.641Å b: 57.422Å c: 61.338Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.248
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cat and Mouse: HIV Transcription in Latency, Immune Evasion and Cure/Remission Strategies.
Delannoy et al. (2019)
1 more

3 mentions without citation

Structure, function and inhibition of critical protein-protein interactions involving mixed lineage leukemia 1 and its fusion oncoproteins.
Li et al. (2021)
2 more