› 5jwl

X-ray diffraction
2.4Å resolution

Factor Inhibiting HIF D201E in Complex with Zn, and Alpha-Ketoglutarate

Released:
DOI: 10.2210/pdb5jwl/pdb
PDB entry 5jwl coloured by chain, front view.
PDB entry 5jwl coloured by chain, side view.
PDB entry 5jwl coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Taabazuing CY, Garman SC, Eron S, Knapp MJ

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand AKG 1 x AKG
Ligand PEG 1 x PEG
Ligand ZN 1 x ZN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41212
Unit cell:
a: 86.131Å b: 86.131Å c: 149.213Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.18 0.226
Expression system: Escherichia coli BL21(DE3)

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