› 5jx1

X-ray diffraction
1.67Å resolution

The neck-linker and alpha 7 helix of Mus musculus KIF3A fused to EB1

Released:
DOI: 10.2210/pdb5jx1/pdb
PDB entry 5jx1 coloured by chain, front view.
PDB entry 5jx1 coloured by chain, side view.
PDB entry 5jx1 coloured by chain, top view.
Source organisms:
Primary publication:
Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips RK, Peter LG, Gilbert SP, Rayment I
J Biol Chem 291 20372-86 (2016)
PMID: 27462072

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF3A; Microtubule-associated protein RP/EB family member 1 Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.92 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28741 (Residues: 352-376; Coverage: 4%)
  • Canonical: Q15691 (Residues: 210-257; Coverage: 18%)
Gene names: Kif3, Kif3a, MAPRE1
Sequence domains: EB1-like C-terminal motif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 45.812Å b: 80.534Å c: 36.834Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Intraflagellar transport trains and motors: Insights from structure.
Webb et al. (2020)
2 more

1 mention without citation

Family-specific Kinesin Structures Reveal Neck-linker Length Based on Initiation of the Coiled-coil.
Phillips et al. (2016)