› 5k11

Electron Microscopy
3.8Å resolution

Cryo-EM structure of isocitrate dehydrogenase (IDH1) in inhibitor-bound state

Released:
DOI: 10.2210/pdb5k11/pdb
PDB entry 5k11 coloured by chain, front view.
PDB entry 5k11 coloured by chain, side view.
PDB entry 5k11 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery.
Merk A, Bartesaghi A, Banerjee S, Falconieri V, Rao P, Davis MI, Pragani R, Boxer MB, Earl LA, Milne JLS, Subramaniam S
Cell 165 1698-1707 (2016)
PMID: 27238019
Related structures: EMD-8193

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 46.33 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: O75874 (Residues: 3-413; Coverage: 99%)
Gene names: IDH1, PICD
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-8193
Expression system: unidentified baculovirus

Quick links

Citations

118 review citations

Unravelling biological macromolecules with cryo-electron microscopy.
Fernandez-Leiro et al. (2016)
117 more

2 mentions without citation

Surgical face masks as a source of emergent pollutants in aquatic systems: Analysis of their degradation product effects in Danio rerio through RNA-Seq.
Sendra et al. (2022)
1 more