Function and Biology Details
Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- WD40 repeat
- G-protein beta WD-40 repeat
- WD40/YVTN repeat-like-containing domain superfamily
- WD40-repeat-containing domain superfamily
- Ubiquitin specific protease, conserved site
- WD40 repeat, conserved site
- Ubiquitin specific protease domain
- Peptidase C19, ubiquitin carboxyl-terminal hydrolase
1 more domain
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-130891 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin carboxyl-terminal hydrolase 12
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 41.26 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
Length: 355 amino acids
Theoretical weight: 41.26 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
- Canonical:
O75317 (Residues: 16-370; Coverage: 96%)
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
WD repeat-containing protein 48
Molecule details ›
Chain: B
Length: 563 amino acids
Theoretical weight: 63.22 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
Sequence domains:
Length: 563 amino acids
Theoretical weight: 63.22 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
- Canonical: Q8TAF3 (Residues: 1-563; Coverage: 83%)
Sequence domains:
WD repeat-containing protein 20
Molecule details ›
Chain: C
Length: 569 amino acids
Theoretical weight: 62.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Length: 569 amino acids
Theoretical weight: 62.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
- Canonical: Q8TBZ3 (Residues: 1-569; Coverage: 100%)
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
